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| Silva, Aline Lourdes Martins; Santana, Marcos Diones Ferreira; Pereira, John César de Jesus; Raimam, Milena Pupo; Albino, Ulisses Brigatto. |
| Arbuscular mycorrhizae microscopy requires differential staining of typical structures. Dyes employed, such as trypan blue, pose risks to health and environment. Alternative dyes such as pen ink and aniline have variable coloring efficiency. In this work, Brachiaria decumbens roots, discolored with caustic soda (NaOH), were stained with açai, annatto, saffron, trypan blue and pen inks. There were significant differences among dyes regarding stained mycorrhizal structures and pictures quality. Acai was considered the best alternative dye, with similar results to trypan blue. |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Recursos Florestais Natural dyes; Soil microbiology; Euterpe oleracea Recursos Florestais; Microbiologia do Solo. Corantes Naturais Microbiologia do solo Técnica. |
| Ano: 2015 |
URL: http://pfb.cnpf.embrapa.br/pfb/index.php/pfb/article/view/798 |
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| Duarte,Lorrane Marques; Bertini,Simone Cristina Braga; Stürmer,Sidney Luiz; Lambais,Marcio Rodrigues; Azevedo,Lucas Carvalho Basilio. |
| ABSTRACT Arbuscular mycorrhizal fungi (AMF) play an important role in plant community productivity and structure, and so studying the factors that affect the diversity and structure of this fungal community is important for understanding their ecology in tropical forests. We investigated AMF spore communities and root colonization under three forest phytophysiognomies (Restinga Forest, REF; Lowland Ombrophilous Dense Forest, LLF; and Montane Ombrophilous Dense Forest, MTF). Spore abundance was lowest in LLF and highest in REF, with no statistical differences relative to MTF. Spore diversity indices and root colonization rates were not statistically different among the phytophysiognomies. However, principal components analysis revealed that AMF community... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Arbuscular mycorrhiza; Glomeromycotina; Hierarchical partitioning; Plant community; Soil attributes; Soil microbiology; Tropical rain forest. |
| Ano: 2019 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-33062019000100050 |
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| Navarrete,Acácio Aparecido; Taketani,Rodrigo Gouvêa; Mendes,Lucas William; Cannavan,Fabiana de Souza; Moreira,Fatima Maria de Souza; Tsai,Siu Mui. |
| The study of the ecology of soil microbial communities at relevant spatial scales is primordial in the wide Amazon region due to the current land use changes. In this study, the diversity of the Archaea domain (community structure) and ammonia-oxidizing Archaea (richness and community composition) were investigated using molecular biology-based techniques in different land-use systems in western Amazonia, Brazil. Soil samples were collected in two periods with high precipitation (March 2008 and January 2009) from Inceptisols under primary tropical rainforest, secondary forest (5-20 year old), agricultural systems of indigenous people and cattle pasture. Denaturing gradient gel electrophoresis of polymerase chain reaction-amplified DNA (PCR-DGGE) using the... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Soil microbial ecology; Soil microbiology; Microbial diversity; Land use changes; Tropical soils. |
| Ano: 2011 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-06832011000500007 |
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| OLIVEIRA, C. A. de; MARRIEL, I. E.; GOMES, E. A.; MATTOS, B. B.; SANTOS, F. C. dos; OLIVEIRA, M. C.; ALVES, V. M. C.. |
| Uma das alternativas promissoras para a agricultura consiste na utilização de rochas naturais, que possuem baixas taxas de solubilização, mas que em associação com microrganismos do solo, denominados solubilizadores de fósforo (MSP), são capazes de suprir, mesmo que parcialmente, a demanda de fósforo (P) pelas plantas. Dentre os estudos de MSP em plantas, poucos se referem a métodos eficientes de aplicação em sementes ou estudos de veiculação/formulação para inoculantes. Neste sentido, o objetivo deste trabalho foi testar a metodologia mais adequada para a aplicação de bactérias solubilizadoras de P com base na avaliação do crescimento e nutrição de plantas de milheto. O delineamento experimental utilizado foi inteiramente casualizado, com quatro... |
| Tipo: Boletim de Pesquisa e Desenvolvimento (INFOTECA-E) |
Palavras-chave: Soil microbiology; Millet.; Microbiologia do solo; Fósforo; Nutrição vegetal; Milheto; Soil biology; Plant nutrition; Phosphorus.. |
| Ano: 2013 |
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/975937 |
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| Rachid,C.T.C.C; Pires,C.A; Leite,D.C.A; Coutinho,H.L.C; Peixoto,R.S; Rosado,A.S; Salton,J; Zanatta,J.A; Mercante,F.M; Angelini,G.A.R; Balieiro,Fabiano de Carvalho. |
| Abstract The sugarcane in Brazil is passing through a management transition that is leading to the abolition of pre-harvest burning. Without burning, large amounts of sugarcane trash is generated, and there is a discussion regarding the utilization of this biomass in the industry versus keeping it in the field to improve soil quality. To study the effects of the trash removal on soil quality, we established an experimental sugarcane plantation with different levels of trash over the soil (0%, 50% and 100% of the original trash deposition) and analyzed the structure of the bacterial and fungal community as the bioindicators of impacts. The soil DNA was extracted, and the microbial community was screened by denaturing gradient gel electrophoresis in two... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Sugarcane trash; Soil quality; Soil microbiology; Fungal community structure; Green cane. |
| Ano: 2016 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822016000200322 |
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